FOLR1 antibody (Biotin)

Catalog No. ABIN99372

Supplier Product No.: 200-106-148-0100

Product Details anti-FOLR1 Antibody (Biotin)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This FOLR1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-106-148-0100

Supplier: Rockland

Purpose: Folate Binding Protein Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].

Characteristics: Synonyms: goat anti-Folate Binding Protein Antibody biotin Conjugation, biotin Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP

Purification: Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Folate Binding Protein [Bovine Milk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Folate Binding Protein has been tested by western blot and is suitable to be assayed against 1.0 μg of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-FOLR1 antibody (Biotin) (ABIN99372)

Kameyama, Motoyama, Tanaka, Yamashita, Higashi, Arima: "Induction of mitophagy-mediated antitumor activity with folate-appended methyl-β-cyclodextrin." in: International journal of nanomedicine, Vol. 12, pp. 3433-3446, (2017) (PubMed).

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1 (FOLR1 Products)

Background: Background: Anti-Folate Binding Protein antibody belongs to the folate receptor family and binds to folate and reduced folic acid derivatives. It further regulates delivery of 5-methyltetrahydrofolate to the cell interior. Folate expresses solely in epithelial tissues, and expression is increased in malignant tissues. Anti-Folate Binding Protein antibody is ideal for researchers investigating Tags & Cell Markers and Cancer research.

Gene ID: 539750

NCBI Accession: NP_001193456

UniProt: P02702

Pathways: Dicarboxylic Acid Transport