GLB1 antibody

Catalog No. ABIN99522

Supplier Product No.: 100-4136

Product Details anti-GLB1 Antibody

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-4136

Supplier: Rockland

Purpose: Beta Galactosidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Beta Galactosidase [E.

Characteristics: Synonyms: rabbit anti-BETA GALACTOSIDASE Antibody, beta-galactosidase, rabbit anti-beta-gal antibody

Purification: Beta-Galactosidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Beta Galactosidase (E.coli)

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Beta-Gal Antibody has been tested by western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:65,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GLB1 antibody (ABIN99522)

Cabrera, Charron, Leib: "Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency." in: Journal of virology, Vol. 92, Issue 13, (2018) (PubMed).

Volckaert, Yuan, Chao, Bell, Sitaula, Szimmtenings, El Agha, Chanda, Majka, Bellusci, Thannickal, Fässler, De Langhe: "Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells." in: Developmental cell, Vol. 43, Issue 1, pp. 48-59.e5, (2017) (PubMed).

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, (2016) (PubMed).

Shearer, Fragoso, Clagett-Dame, McCaffery: "Astrocytes as a regulated source of retinoic acid for the brain." in: Glia, (2012) (PubMed).

Perry, He, Sugimura, Grindley, Haug, Ding, Li: "Cooperation between both Wnt/{beta}-catenin and PTEN/PI3K/Akt signaling promotes primitive hematopoietic stem cell self-renewal and expansion." in: Genes & development, Vol. 25, Issue 18, pp. 1928-42, (2011) (PubMed).

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Beta Galactosidase (GLB1 Products)

Background: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process