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General |
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Antigen | Keratin 18 (KRT18) Antibodies |
Reactivity | Cow (Bovine), Human Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This KRT18 antibody is un-conjugated Alternatives |
Application |
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Alternatives
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3 references available |
Supplier | Log in to see |
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN125989) Images |
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Specificity | Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18, formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine, negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA. |
Purification | Protein A affinity chromatography |
Immunogen | Cytoskeletal preparation from tumor cell line MCF-7. |
Clone | Ks18-27 |
Isotype | IgG1 |
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN125989) Images back to top |
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Antigen | |
Alternative Name | Cytokeratin 18 (KRT18 Antibody Abstract) |
Background | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18 |
Gene ID | 3875 |
NCBI Accession | NP_000215 |
UniProt | P05783 |
Pathways | Apoptosis, Caspase Cascade in Apoptosis |
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN125989) Images back to top |
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Application Notes |
Immunohistochemistry on frozen sections: 1: 10, 1 h at RT. Cytological material. Immunoblotting. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN125989) Images back to top |
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Reconstitution | Restore in 1 mL dist. water |
Buffer | PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | This product is photosensitive and should be protected from light |
Storage | 4 °C |
Storage Comment | Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! |
References for anti-KRT18 antibody (ABIN125989)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top |
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Background publications |
Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 Moll, Achtstätter, Becht, Balcarova-Ständer, Ittensohn, Franke: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, 1988 Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 |