anti-Cow (Bovine) KRT18 antibody for Enzyme Immunoassay

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Cow (Bovine), Human
1123
245
235
136
91
91
62
58
44
43
39
34
29
29
29
27
25
24
6
4
3
2
2
1
1
1
1
Host
Mouse
852
300
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
58
51
27
20
20
18
16
15
13
13
13
12
12
12
12
11
11
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
4
3
3
3
3
3
3
3
3
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
712
553
522
454
445
284
215
171
157
117
45
41
18
14
10
9
3
3
2
1
1
1
Options
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Supplier Product No.
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Pubmed
3 references available
Catalog No. ABIN125989
$ 368.50
Plus shipping costs $45.00

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Cow (Bovine), Human
(1123), (245), (235), (136), (91), (91), (62), (58), (44), (43), (39), (34), (29), (29), (29), (27), (25), (24), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(852), (300), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(58), (51), (27), (20), (20), (18), (16), (15), (13), (13), (13), (12), (12), (12), (12), (11), (11), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(712), (553), (522), (454), (445), (284), (215), (171), (157), (117), (45), (41), (18), (14), (10), (9), (3), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN125989) Images
Specificity Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18, formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine, negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA.
Purification Protein A affinity chromatography
Immunogen Cytoskeletal preparation from tumor cell line MCF-7.
Clone Ks18-27
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN125989) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
NCBI Accession NP_000215
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN125989) Images back to top
Application Notes Immunohistochemistry on frozen sections: 1: 10, 1 h at RT. Cytological material. Immunoblotting. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN125989) Images back to top
Reconstitution Restore in 1 mL dist. water
Buffer PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-KRT18 antibody (ABIN125989)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990

Moll, Achtstätter, Becht, Balcarova-Ständer, Ittensohn, Franke: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, 1988

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983