anti-Dog (Canine) KRT18 antibody for Simple Western

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Cat (Feline), Dog (Canine), Human
864
196
196
117
86
61
59
46
42
36
32
27
27
27
26
15
11
9
6
4
3
2
1
1
1
1
Host
Mouse
603
290
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
48
42
18
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
4
4
3
3
3
3
3
3
3
3
3
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
587
381
357
352
265
206
171
103
87
80
45
40
18
14
11
3
2
1
1
1
Options
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Supplier Product No.
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Catalog No. ABIN4302743
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General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Cat (Feline), Dog (Canine), Human
(864), (196), (196), (117), (86), (61), (59), (46), (42), (36), (32), (27), (27), (27), (26), (15), (11), (9), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(603), (290), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(48), (42), (18), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
(587), (381), (357), (352), (265), (206), (171), (103), (87), (80), (45), (40), (18), (14), (11), (3), (2), (1), (1), (1)
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling Images
Specificity This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
No Cross-Reactivity Rat (Rattus)
Purification Protein A or G purified
Immunogen Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431.
Clone DE-K18
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Gene Symbol: KRT18
Molecular Weight Theoretical MW: 45 kDa
Gene ID 3875
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Application Notes Simple Western 10 μg/mL, Flow Cytometry 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 μg/mL, Immunofluorescence 1 - 2 μg/mLHu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 2 - 4 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
Format Liquid
Buffer 10 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.