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|Antigen||Keratin 18 (KRT18) Antibodies|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN371615) Images
|Specificity||This antibody reacts specifically specifically with Keratin 18 and recombinant N-terminal Keratin 18.|
|Characteristics||Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18|
|Immunogen||Recombinant N-terminal fragment of keratin 18.|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN371615) Images back to top
|Alternative Name||Cytokeratin 18 (KRT18 Antibody Abstract)|
|Background||Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30 % sequence homology. (Ref.1-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48 kDa. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8. (Ref.6-8) Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN371615) Images back to top
ELISA: 1/5,000-1/20,000. Western Blot: 1/2,000-1/4,000. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): 10 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN371615) Images back to top
|Buffer||100 mM Borate buffered saline, pH 8.0 without preservatives or amine-containing buffer salts.|
Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-KRT18 antibody (ABIN371615)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Ku, Darling, Krams, Esquivel, Keeffe, Sibley, Lee, Wright, Omary: "Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 10, pp. 6063-8, 2003
Kulesh, Oshima: "Complete structure of the gene for human keratin 18." in: Genomics, Vol. 4, Issue 3, pp. 339-47, 1989
Steinert, Roop: "Molecular and cellular biology of intermediate filaments." in: Annual review of biochemistry, Vol. 57, pp. 593-625, 1988
Schiller, Franke, Geiger: "A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells." in: The EMBO journal, Vol. 1, Issue 6, pp. 761-9, 1984
Hanukoglu, Fuchs: "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins." in: Cell, Vol. 33, Issue 3, pp. 915-24, 1983
Tseng, Jarvinen, Nelson, Huang, Woodcock-Mitchell, Sun: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983
Fuchs, Coppock, Green, Cleveland: "Two distinct classes of keratin genes and their evolutionary significance." in: Cell, Vol. 27, Issue 1 Pt 2, pp. 75-84, 1982