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|Antigen||Keratin 18 (KRT18) Antibodies|
|Reactivity||Cat (Feline), Cow (Bovine), Dog (Canine), Ferret, Goat, Hamster, Horse (Equine), Human, Marmoset, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling Images
|Specificity||This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.|
|No Cross-Reactivity||Chicken, Xenopus laevis|
|Purification||Purified by Protein A/G|
|Immunogen||Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431|
|Plasmids, Primers & others|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling Images back to top
|Alternative Name||KRT18 (KRT18 Antibody Abstract)|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Positive Control: MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
|Buffer||10 mM PBS with 0.05 % BSA & 0.05 % azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-80 °C|
|Storage Comment||Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.|
|Expiry Date||24 months|