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|Antigen||tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This YWHAZ antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-YWHAZ AntibodyTarget Details YWHAZ Application Details Handling References for anti-YWHAZ antibody (ABIN272122) Images
|Specificity||This antibody detects endogenous levels of 14-3-3 ζ/δ protein. (region surrounding Trp228)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
|Plasmids, Primers & others|
Target Details YWHAZProduct Details anti-YWHAZ Antibody Application Details Handling References for anti-YWHAZ antibody (ABIN272122) Images back to top
|Alternative Name||14-3-3 Protein zeta/delta (YWHAZ Antibody Abstract)|
|Background||14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ|
|Molecular Weight||approx. 28 kDa|
|Pathways||Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location|
Application DetailsProduct Details anti-YWHAZ Antibody Target Details YWHAZ Handling References for anti-YWHAZ antibody (ABIN272122) Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-YWHAZ Antibody Target Details YWHAZ Application Details References for anti-YWHAZ antibody (ABIN272122) Images back to top
|Buffer||Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-YWHAZ antibody (ABIN272122)Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling Images back to top
|Product cited in:||
Nagappan, Park, Park, Hong, Yumnam, Lee, Kim, Kim, Lee, Lee, Cho, Lee, Won, Cho, Kim: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." in: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, 2013
ImagesProduct Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling References for anti-YWHAZ antibody (ABIN272122) back to top