Protein G Resin Bead
- Protein G
- Affinity Chromatography (AC), Purification (Purif)
- Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media.
The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
- Number of IgG binding sites per ligand: 3
- MW of ligand: Approximately 22 kDa
- PI of ligand: 4.69
- Degree of substitution: Approximately 2 mg protein G/ml settled resin
- Static binding capacity: >20 mg sheep IgG/ml settled resin
- Matrix spherical: agarose, 4% cross-linked
- Average particle size: 90 μm (45-165 μm)
- Bead Ligand
- Protein G
- Bead Matrix
- Agarose beads
- Bead Size
- 90 µm
High temperature heating is not recommended. The agarose melts above 65°C.
- For Research Use only
- 1X PBS containing 20% ethanol
- Handling Advice
- High temperature heating is not recommended. The agarose melts above 65°C.
- 4 °C
- Storage Comment
- Store regenerated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
- Expiry Date
- 18 months
Tukaj, Grüner, Tukaj, Zillikens, Kasperkiewicz: "Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid." in: Journal of the European Academy of Dermatology and Venereology : JEADV, 2015 (PubMed).
Hakimi, Goto, Suganuma, Angeles, Kawai, Inoue, Kawazu: "Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis." in: Experimental parasitology, Vol. 154, pp. 62-6, 2015 (PubMed).
Ringel, Probst, Dammeyer, Buchmeier, Jänsch, Wissing, Tinnefeld, Mendel, Jockusch, Kruse: "Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa." in: Fungal genetics and biology : FG & B, Vol. 80, pp. 10-8, 2015 (PubMed).
Tian, von Dahl, Liu, Friso, van Wijk, Klessig: "The combined use of photoaffinity labeling and surface plasmon resonance-based technology identifies multiple salicylic acid-binding proteins." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 6, pp. 1027-38, 2014 (PubMed).
Muth, Schütze, Hain, Yagita, Schild, Probst: "A CD40/CD40L feedback loop drives the breakdown of CD8(+) T-cell tolerance following depletion of suppressive CD4(+) T cells." in: European journal of immunology, Vol. 44, Issue 4, pp. 1099-107, 2014 (PubMed).
Armitage, OMeara, Harvie, Timms, Blumberg, Beagley: "Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens." in: Immunology and cell biology, Vol. 92, Issue 5, pp. 417-26, 2014 (PubMed).
Kan, Adjobo-Hermans, Burroughs, Faibis, Malik, Tall, Smrcka: "M3 muscarinic receptor interaction with phospholipase C ?3 determines its signaling efficiency." in: The Journal of biological chemistry, Vol. 289, Issue 16, pp. 11206-18, 2014 (PubMed).
Zilio, Codlin, Vashisht, Bitton, Head, Wohlschlegel, Bähler, Boddy: "A novel histone deacetylase complex in the control of transcription and genome stability." in: Molecular and cellular biology, Vol. 34, Issue 18, pp. 3500-14, 2014 (PubMed).
Zhao, Guo, Ma, Xing, Wang: "Generation and characterization of polyclonal antibody against part of immunoglobulin constant heavy ? chain of goose." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 33, Issue 4, pp. 287-90, 2014 (PubMed).
Zhang, Sun, Fettke, Schöttler, Ramsden, Fernie, Lim: "Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development." in: FEBS letters, Vol. 588, Issue 20, pp. 3726-31, 2014 (PubMed).
Feng, Jiang, Wu, Marlow: "Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish." in: Developmental biology, Vol. 395, Issue 2, pp. 268-86, 2014 (PubMed).
Tubon, Zhang, Friedman, Jin, Gonzales, Zhou, Drier, Gerstner, Paulson, Fropf, Yin: "dCREB2-mediated enhancement of memory formation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 17, pp. 7475-87, 2013 (PubMed).
Short, von Köckritz-Blickwede, Langereis, Chew, Job, Armitage, Hatcher, Fujihashi, Reading, Hermans, Wijburg, Diavatopoulos: "Antibodies mediate formation of neutrophil extracellular traps in the middle ear and facilitate secondary pneumococcal otitis media." in: Infection and immunity, Vol. 82, Issue 1, pp. 364-70, 2013 (PubMed).
Bikkavilli, Malbon: "Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2." in: Journal of cell science, Vol. 125, Issue Pt 10, pp. 2446-56, 2012 (PubMed).
Muth, Schütze, Schild, Probst: "Release of dendritic cells from cognate CD4+ T-cell recognition results in impaired peripheral tolerance and fatal cytotoxic T-cell mediated autoimmunity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 23, pp. 9059-64, 2012 (PubMed).
Quinones, Danowski, Devaraj, Singh, Ligon: "The posttranslational modification of tubulin undergoes a switch from detyrosination to acetylation as epithelial cells become polarized." in: Molecular biology of the cell, Vol. 22, Issue 7, pp. 1045-57, 2011 (PubMed).
Staron, Wu, Hong, Stojanovic, Du, Bona, Liu, Li: "Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex." in: Blood, Vol. 117, Issue 26, pp. 7136-44, 2011 (PubMed).
Jiang, Zhang, Shen, Wang, Jiang: "Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption." in: Talanta, Vol. 82, Issue 3, pp. 1003-9, 2010 (PubMed).
Chiranand, McLeod, Zhou, Lynn, Vega, Myers, Yates, Lorenz, Gustin: "CTA4 transcription factor mediates induction of nitrosative stress response in Candida albicans." in: Eukaryotic cell, Vol. 7, Issue 2, pp. 268-78, 2008 (PubMed).
- Tukaj, Grüner, Tukaj, Zillikens, Kasperkiewicz: "Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid." in: Journal of the European Academy of Dermatology and Venereology : JEADV, 2015 (PubMed).
- Protein G
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Broad IgG binding spectrum
Binding specificity complements of protein A
No specific albumin binding
Optimized homogeneous recombinant ligand