GFP-Catcher

Catalog No. ABIN5311508

Product Details

Key Features:

• High-affinity single-domain antibody
• Recognizes GFP and many GFP derivatives
• Bead size 50-150 μm

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Camelid (Camelidae)

Application: Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)

Purpose: GFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

Sample Type: Cell Extracts

Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Cross-Reactivity (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Characteristics: GFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer.
GFP-Catcher thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.
GFP-Catcher immobilizes a wide range of GFP derivatives.
GFP-Catcher is compatible not only with physiological buffers but also with high stringency buffers.
GFP-Catcher thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Components: 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody

Material not included: wash buffers, columns, tubes

Bead Ligand: Antibody

Bead Matrix: Agarose beads

Bead Size: 90 μm

Application Details

Application Notes: Coating: sdAb anti-GFP clone 1H1
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Capacity: > 4 μg GFP per μl of packed beads (= 2 μL of slurry)
Buffer Compatibility:

• Common buffer substances at pH 5 to 9
• 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
• 4 M urea
• 10 mM DTT, 10 mM 2-Mercaptoethanol
• RNAse A, DNAse I, Benzonase, protease inhibitors

Restrictions: For Research Use only

Handling

Buffer: 50 % slurry in PBS containing 20 % Ethanol

Storage: 4 °C

Storage Comment: Store at 4 °C, do not freeze

Expiry Date: 12 months

Target Details

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein Bead, gfp Bead

Target Type: Viral Protein