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- Target See all IgG2a products
- IgG2a
- Reactivity
- Mouse
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Host
- HEK-293 Cells
- Application
- Isotype Control (IsoC)
- Characteristics
- This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa under reducing (R) condition, and 50-60 kDa under non-reducing (NR) condition (SDS-PAGE).
- Purity
- >90 % as determined by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Isotype
- IgG2a
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Mouse IgG2a isotype control (APC)
FACS Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (FITC)FACS Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (PE)FACS Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, IsoC, IF IgG2a FITC
Mouse IgG2a isotype controlFACS, IsoC IgG2a unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Buffer
- 50 mM Tris, 100 mM Glycine, pH 7.5
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
- Background
- Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
- Molecular Weight
- 26.4 kDa
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