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Mouse IgG2a Isotype Control

FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Catalog No. ABIN2749088
  • Target See all IgG2a products
    IgG2a
    Host
    • 170
    • 66
    • 3
    • 1
    Mouse
    Clonality
    • 171
    • 2
    • 1
    Monoclonal
    Application
    Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
    No Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
    Clone
    • 29
    • 13
    • 13
    • 12
    • 10
    • 10
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    MOPC-173
    Isotype
    IgG2a kappa
  • Application Notes
    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Khoddami, Cairns: "Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP." in: Nature protocols, Vol. 9, Issue 2, pp. 337-61, (2014) (PubMed).

    Gupta, Gylling, Alonso, Sugimori, Ianakiev, Xiong, Arnaout: "The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18." in: Blood, Vol. 109, Issue 8, pp. 3513-20, (2007) (PubMed).

    Fougereau, Bourgois, de Preval, Rocca-Serra, Schiff: "The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications." in: Annales d'immunologie, Vol. 127, Issue 5, pp. 607-31, (1977) (PubMed).

    Baumal, Scharff: "Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 116, Issue 1, pp. 65-74, (1976) (PubMed).

  • Target
    IgG2a
    Abstract
    IgG2a Products
    Synonyms
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Target Type
    Antibody
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