IL12B ELISA Kit (Interleukin 12b)

Details for Product IL12B ELISA Kit No. ABIN1672845, Supplier: Log in to see
Antigen
  • CLMF
  • CLMF2
  • IL-12B
  • NKSF
  • NKSF2
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • IL-12p40
  • IL-12
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • IL-12.p40
  • IL12p40
  • IL12B
  • CLMF p40
  • interleukin 12B
  • interleukin 12b
  • interleukin 12Ba
  • IL12B
  • Il12b
  • il12ba
Epitope
AA 23-335
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
38
19
13
10
10
8
8
7
6
5
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-12(p40)
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: sf21
Immunogen sequence: M23-S335
Specificity Expression system for standard: sf21
Immunogen sequence: M23-S335
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Alternative Name IL12B (IL12B ELISA Kit Abstract)
Background

Protein Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Background: Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40- kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35- kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

Synonyms: Interleukin-12 subunit beta,IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40,Il12b,

Full Gene Name: Interleukin-12 subunit beta

Cellular Localisation: Secreted.
Gene ID 16160
UniProt P43432
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the type I cytokine receptor family. Type 3 subfamily.

Plate Pre-coated
Protocol mouse IL-12(p40) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(p40) has been precoated onto 96-well plates. Standards(sf21, M23-S335) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p40) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(p40) amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(p40) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(p40) standard solution and each sample be measured in duplicate.

Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 136, Standard deviation: 8.3, CV(%): 6.1
  • Sample 2: n=16, Mean(pg/ml): 390, Standard deviation: 20.28, CV(%): 5.2
  • Sample 3: n=16, Mean(pg/ml): 683, Standard deviation: 31.42, CV(%): 4.6,
  • Sample 1: n=24, Mean(pg/ml): 165, Standard deviation: 11.55, CV(%): 7
  • Sample 2: n=24, Mean(pg/ml): 446, Standard deviation: 30.33, CV(%): 6.8
  • Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 40.1, CV(%): 5.6
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Supplier Images
ELISA image for Interleukin 12b (IL12B) ELISA Kit (ABIN1672845) Mouse IL-12(p40) PicoKine ELISA Kit standard curve
Product cited in: Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014 (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014 (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014 (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014 (PubMed).

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013 (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012 (PubMed).

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011 (PubMed).

Background publications Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain." in: Nature, Vol. 421, Issue 6924, pp. 744-8, 2003 (PubMed).

Becher, Durell, Noelle: "Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12." in: The Journal of clinical investigation, Vol. 110, Issue 4, pp. 493-7, 2002 (PubMed).

Wolf, Sieburth, Sypek: "Interleukin 12: a key modulator of immune function." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 2, pp. 154-68, 1994 (PubMed).

Sieburth, Jabs, Warrington, Li, Lasota, LaForgia, Kelleher, Huebner, Wasmuth, Wolf: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992 (PubMed).

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