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Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit ELISA Kit

TNFRSF4 Reactivity: Human AA 29-216 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant
Catalog No. ABIN1672897
$369.00
Plus shipping costs $45.00
96 tests
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Delivery in 4 to 6 Business Days
  • Target
    Binding Specificity
    AA 29-216
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF4/OX40
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: L29-A216
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L29-A216
  • Target
    Alternative Name
    TNFRSF4 (TNFRSF4 ELISA Kit Abstract)
    Background

    Protein Function: Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity. .

    Background: Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.

    Synonyms: Tumor necrosis factor receptor superfamily member 4,ACT35 antigen,OX40L receptor,TAX transcriptionally-activated glycoprotein 1 receptor,CD134,TNFRSF4,TXGP1L,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 4

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    7293
    UniProt
    P43489
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 4 TNFR-Cys repeats.

    Plate
    Pre-coated
    Protocol
    human TNFRSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF4 has been precoated onto 96-well plates. Standards(NSO, L29-A216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF4 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFRSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF4 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 11.1, CV(%): 4.7
    • Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 42.5, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 1245, Standard deviation: 79.68, CV(%): 6.4,
    • Sample 1: n=24, Mean(pg/ml): 250, Standard deviation: 13.25, CV(%): 5.3
    • Sample 2: n=24, Mean(pg/ml): 838, Standard deviation: 50.28, CV(%): 6
    • Sample 3: n=24, Mean(pg/ml): 1335, Standard deviation: 96.12, CV(%): 7.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
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