Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit ELISA Kit
- Detection Method
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
Synonyms: ACT35, ACT35 antigen, CD134, CD134 antigen, Lymphoid activation antigene ACT35, OX40, OX40 antigen, OX40 cell surface antigen, OX40 homologue, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TNR4_HUMAN, Tumor necrosis factor receptor superfamily member 4, TXGP1L
Background: Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.
Gene Name: TNFRSF4
Production: Natural and recombinant mouse TNFRSF4. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: V20-P211
- Application Notes
Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFRSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFRSF4 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- Sample Volume
- 100 μL
- For Research Use only
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Alternative Name
- TNFRSF4 - OX40 (TNFRSF4 ELISA Kit Abstract)
- Gene ID
- NCBI Accession
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints