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TGFBI ELISA Kit

TGFBI Reactivity: Human AA 24-683 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Pubmed (1 reference)
Catalog No. ABIN1889371
$499.00
Plus shipping costs $45.00
96 tests
Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target See all TGFBI ELISA Kits
    TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
    Binding Specificity
    AA 24-683
    Reactivity
    • 7
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TGFBI/betaIG-H3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: G24-H683
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: G24-H683
    Featured
    Discover our best selling TGFBI ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 EMI domain.

    Tissue Specificity: Highly expressed in the corneal epithelium. .

    Plate
    Pre-coated
    Protocol
    human TGFBI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBI has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBI amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TGFBI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGFBI standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 672, Standard deviation: 30.24, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 1913, Standard deviation: 89.9, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 2844, Standard deviation: 150.7, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 764, Standard deviation: 39.7, CV(%): 5.2
    • Sample 2: n=24, Mean(pg/ml): 1860, Standard deviation: 106.02, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 2927, Standard deviation: 184.4, CV(%): 6.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Jin, Wan, Chen, Chen, Zhang, Deng, Zhang, Xiong, Xin: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).

  • Target See all TGFBI ELISA Kits
    TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
    Alternative Name
    TGFBI (TGFBI Products)
    Synonyms
    TGFBI ELISA Kit, BIGH3 ELISA Kit, CDB1 ELISA Kit, CDG2 ELISA Kit, CDGG1 ELISA Kit, CSD ELISA Kit, CSD1 ELISA Kit, CSD2 ELISA Kit, CSD3 ELISA Kit, EBMD ELISA Kit, LCD1 ELISA Kit, 68kDa ELISA Kit, AI181842 ELISA Kit, AI747162 ELISA Kit, Beta-ig ELISA Kit, big-h3 ELISA Kit, Big-h3 ELISA Kit, MP70 ELISA Kit, MP78 ELISA Kit, hm:zeh0933 ELISA Kit, wu:fa13f05 ELISA Kit, zgc:110495 ELISA Kit, transforming growth factor beta induced ELISA Kit, transforming growth factor, beta induced ELISA Kit, transforming growth factor, beta-induced ELISA Kit, TGFBI ELISA Kit, Tgfbi ELISA Kit, tgfbi ELISA Kit
    Background

    Protein Function: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

    Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

    Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Kerato-epithelin,RGD-containing collagen-associated protein,RGD-CAP,TGFBI,BIGH3,

    Full Gene Name: Transforming growth factor-beta-induced protein ig-h3

    Cellular Localisation: Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface.
    Gene ID
    7045
    UniProt
    Q15582
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