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TGFBI ELISA Kit

TGFBI Reactivity: Mouse AA 24-683 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Pubmed (1 reference)
Catalog No. ABIN2859321
$499.00
Plus shipping costs $45.00
96 tests
Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target See all TGFBI ELISA Kits
    TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
    Binding Specificity
    AA 24-683
    Reactivity
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse beta IG-H3/TGFBI
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    NSO, G24-H683
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <5pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: G24-H683
    Featured
    Discover our best selling TGFBI ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed in heart, kidney, liver, skeletal muscle, testis, thyroid and uterus (PubMed:8024701). .

    Plate
    Pre-coated
    Protocol
    mouse beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for beta IG-H3 has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse beta IG-H3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse βIG-H3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse βIG-H3 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 447, Standard deviation: 28.61, CV(%): 6.4
    • Sample 2: n=16, Mean(pg/ml): 1253, Standard deviation: 53.88, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 2346, Standard deviation: 115, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 39.15, CV(%): 8.7
    • Sample 2: n=24, Mean(pg/ml): 1238, Standard deviation: 64.38, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 2625, Standard deviation: 144.4, CV(%): 5.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Ge, Yu, Liu, Cong, Liu, Wang, Zhou, Lin: "Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3498-506, (2016) (PubMed).

  • Target See all TGFBI ELISA Kits
    TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
    Alternative Name
    TGFBI (TGFBI Products)
    Synonyms
    TGFBI ELISA Kit, BIGH3 ELISA Kit, CDB1 ELISA Kit, CDG2 ELISA Kit, CDGG1 ELISA Kit, CSD ELISA Kit, CSD1 ELISA Kit, CSD2 ELISA Kit, CSD3 ELISA Kit, EBMD ELISA Kit, LCD1 ELISA Kit, 68kDa ELISA Kit, AI181842 ELISA Kit, AI747162 ELISA Kit, Beta-ig ELISA Kit, big-h3 ELISA Kit, Big-h3 ELISA Kit, MP70 ELISA Kit, MP78 ELISA Kit, hm:zeh0933 ELISA Kit, wu:fa13f05 ELISA Kit, zgc:110495 ELISA Kit, transforming growth factor beta induced ELISA Kit, transforming growth factor, beta induced ELISA Kit, transforming growth factor, beta-induced ELISA Kit, TGFBI ELISA Kit, Tgfbi ELISA Kit, tgfbi ELISA Kit
    Background

    Protein Function: Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity). .

    Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

    Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Tgfbi,

    Full Gene Name: Transforming growth factor-beta-induced protein ig-h3

    Cellular Localisation: Secreted . Secreted, extracellular space, extracellular matrix . May be associated both with microfibrils and with the cell surface..
    Gene ID
    21810
    UniProt
    P82198
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