Thioredoxin (TXN) ELISA Kit

Details for Product No. ABIN1889406
Antigen
  • TRDX
  • TRX
  • TRX1
  • ADF
  • AW550880
  • Trx1
  • Txn
  • trx
  • TXN
  • SCM1.18
  • trxA3
  • thioredoxin
  • TR-1
  • Trx-1
  • thioredoxin
  • thioredoxin 1
  • thioredoxin L homeolog
  • similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544)
  • thioredoxin pseudogene
  • cytosolic thioredoxin Trx1
  • TXN
  • Txn1
  • txn.L
  • txn
  • trx
  • SCO0885
  • PHG25ORF099c
  • LOC100349046
  • Txn
  • trx1
Epitope
AA 2-105
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
9
7
7
5
3
3
3
3
2
2
2
2
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thioredoxin
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin)
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: E.coli
Immunogen sequence: V2-V105
Specificity Expression system for standard: E.coli
Immunogen sequence: V2-V105
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Antigen
Alternative Name TXN (TXN ELISA Kit Abstract)
Background

Protein Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

Synonyms: Thioredoxin,Trx,ATL-derived factor,ADF,Surface-associated sulphydryl protein,SASP,TXN,TRDX, TRX, TRX1,

Full Gene Name: Thioredoxin

Cellular Localisation: Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
Gene ID 7295
UniProt P10599
Pathways Carbohydrate Homeostasis, Cell RedoxHomeostasis
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the thioredoxin family.

Plate Pre-coated
Protocol human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Thioredoxin has been precoated onto 96-well plates. Standards(E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.

Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 0.89, Standard deviation: 0.05, CV(%): 5.6
  • Sample 2: n=16, Mean(ng/ml): 3.62, Standard deviation: 0.17, CV(%): 4.7
  • Sample 3: n=16, Mean(ng/ml): 6.83, Standard deviation: 0.37, CV(%): 5.4,
  • Sample 1: n=24, Mean(ng/ml): 0.87, Standard deviation: 0.07, CV(%): 8.0
  • Sample 2: n=24, Mean(ng/ml): 3.85, Standard deviation: 0.24, CV(%): 6.2
  • Sample 3: n=24, Mean(ng/ml): 7.12, Standard deviation: 0.41, CV(%): 5.8
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Supplier Images
ELISA image for Thioredoxin (TXN) ELISA Kit (ABIN1889406) Human Thioredoxin PicoKine ELISA Kit standard curve
Product cited in: Csősz, Lábiscsák, Kalló, Márkus, Emri, Szabó, Tar, Tőzsér, Kiss, Márton: "Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers." in: PLoS ONE, Vol. 12, Issue 5, pp. e0177282, 2017 (PubMed).

Background publications Arnér, Holmgren: "Physiological functions of thioredoxin and thioredoxin reductase." in: European journal of biochemistry / FEBS, Vol. 267, Issue 20, pp. 6102-9, 2000 (PubMed).

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