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Noradrenaline/Norepinephrine ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Chemical .
Catalog No. ABIN2648798

Quick Overview for Noradrenaline/Norepinephrine ELISA Kit (ABIN2648798)

Target

Noradrenaline/Norepinephrine

Reactivity

  • 2
  • 1
  • 1
Chemical

Detection Method

Colorimetric

Application

ELISA

Sample Type

Urine, Plasma
  • Sensitivity

    Urine: 0.85 ng/ml, Plasma: 43 pg/ml

    Characteristics

    Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Sample Volume

    15 μL

    Plate

    Pre-coated

    Protocol

    The competitive Noradrenaline (Norepinephrine) ELISA Kit uses the microtiter plate format. Noradrenaline is bound to the solid phase of the microtiter plate. Acylated noradrenaline from the sample and solid phase bound noradrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase noradrenaline is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase noradrenaline is inversely proportional to the noradrenaline concentration of the sample.

    Restrictions

    For Research Use only
  • Storage

    4 °C
  • Target

    Noradrenaline/Norepinephrine

    Alternative Name

    Noradrenaline (Norepinephrine)

    Target Type

    Chemical
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