Noradrenaline/Norepinephrine ELISA Kit

Catalog No. ABIN2648799

Product Details

Target: Noradrenaline/Norepinephrine

Reactivity: Mouse, Rat

Detection Method: Colorimetric

Application: ELISA

Sample Type: Cell Culture Cells, Plasma, Tissue Homogenate

Sensitivity: 1 pg/mL

Characteristics: Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Plate: Pre-coated

Protocol: The competitive Noradrenaline (Norepinephrine) High Sensitive ELISA kit uses the microtitre plate format. Noradrenaline is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Noradrenaline/Norepinephrine

Alternative Name: Noradrenaline (Norepinephrine)

Target Type: Chemical