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FAS ELISA Kit

FAS Reactivity: Human AA 17-173 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Pubmed (1 reference)
Catalog No. ABIN411269
$499.00
Plus shipping costs $45.00
96 tests
Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target See all FAS ELISA Kits
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Binding Specificity
    AA 17-173
    Reactivity
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Soluble FAS
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: R17-N173
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <3pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: R17-N173
    Featured
    Discover our best selling FAS ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 death domain.

    Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. .

    Plate
    Pre-coated
    Protocol
    human FAS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS has been precoated onto 96-well plates. Standards(NSO, R17-N173) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FAS amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FAS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human FAS standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 138, Standard deviation: 5.80, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 828, Standard deviation: 25.67, CV(%): 3.1
    • Sample 3: n=16, Mean(pg/ml): 1507, Standard deviation: 70.83, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 10.95, CV(%): 7.5
    • Sample 2: n=24, Mean(pg/ml): 839, Standard deviation: 45.31, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 1512, Standard deviation: 83.16, CV(%): 5.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

  • Target See all FAS ELISA Kits
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Alternative Name
    FAS (FAS Products)
    Synonyms
    ALPS1A ELISA Kit, APO-1 ELISA Kit, APT1 ELISA Kit, CD95 ELISA Kit, FAS1 ELISA Kit, FASTM ELISA Kit, TNFRSF6 ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, APT1LG1 ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, AI196731 ELISA Kit, APO1 ELISA Kit, TNFR6 ELISA Kit, Tnfrsf6 ELISA Kit, lpr ELISA Kit, Fas cell surface death receptor ELISA Kit, Fas ligand ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta ELISA Kit, Fas (TNF receptor superfamily member 6) ELISA Kit, FAS ELISA Kit, FASLG ELISA Kit, Fasl ELISA Kit, YWHAZ ELISA Kit, Fas ELISA Kit, fas ELISA Kit
    Background

    Protein Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). .

    Background: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48- kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

    Synonyms: Tumor necrosis factor receptor superfamily member 6,Apo-1 antigen,Apoptosis-mediating surface antigen FAS,FASLG receptor,CD95,FAS,APT1, FAS1, TNFRSF6,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 6

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    355
    UniProt
    P25445
    Pathways
    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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