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TNFSF9 ELISA Kit (Tumor Necrosis Factor (Ligand) Superfamily, Member 9) ELISA Kit

TNFSF9 Reactivity: Human Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4881698
$501.60
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  • Target
    TNFSF9
    Reactivity
    Human
    • 3
    • 2
    • 1
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.2-50 ng/mL
    Minimum Detection Limit
    0.2 ng/mL
    Application
    ELISA
    Purpose
    Human 41BB Ligand (TNFSF9) ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects human 4-1BB Ligand. Other species not determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling TNFSF9 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target
    TNFSF9
    Alternative Name
    4-1BB Ligand///TNFSF9 (TNFSF9 ELISA Kit Abstract)
    Synonyms
    TNFSF9, 4-1BB-L, 4-1BBL, AI848817, Cd137l, Ly63l, CD137L, TNF superfamily member 9, tumor necrosis factor (ligand) superfamily, member 9, Tnfsf9, LOC476729, TNFSF9
    Gene ID
    8744
    UniProt
    P41273
    Pathways
    Activated T Cell Proliferation, Cancer Immune Checkpoints
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