PDGFD ELISA Kit (Platelet Derived Growth Factor D)

Details for Product PDGFD ELISA Kit No. ABIN5657899, Supplier: Log in to see
Antigen
  • IEGF
  • SCDGF-B
  • SCDGFB
  • Scdgfb
  • rSCDGF-B
  • PDGFD
  • 1110003I09Rik
  • platelet derived growth factor D
  • platelet-derived growth factor, D polypeptide
  • PDGFD
  • Pdgfd
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
16
9
2
2
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.625 ng/mL - 40 ng/mL
Minimum Detection Limit
0.625 ng/mL
Application
ELISA
Options
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Sample Type Plasma
Analytical Method Quantitative
Detection Method Chemiluminescent
Specificity This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor D (PDGFD). No significant cross-reactivity or interference between Platelet Derived Growth Factor D (PDGFD) and analogues was observed.
Sensitivity 0.03 ng/mL
Alternative Name Platelet Derived Growth Factor D (PDGFD ELISA Kit Abstract)
Background

Gene Name: Platelet Derived Growth Factor D

Gene Aliases: PDGF-D, IEGF, SCDGF-B, Spinal Cord Derived Growth Factor B, Iris-expressed growth factor, Platelet-derived growth factor D, receptor-binding form/latent form

Gene ID 80310
UniProt Q9GZP0
Pathways RTK Signaling, Platelet-derived growth Factor Receptor Signaling
Comment

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time 2 - 3 h
Plate Pre-coated
Protocol The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor D (PDGFD) level in the sample or standard.,
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Handling Advice Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage 4 °C,-20 °C
Storage Comment -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date 4-8 months
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