Interleukin 8 IQ-ELISA Kit (IL8)

Details for Product IL8 IQ-ELISA Kit No. ABIN6244364
Antigen
  • CXCL8
  • GCP-1
  • GCP1
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MONAP
  • NAF
  • NAP-1
  • NAP1
  • IL-8
  • AMCF-I
  • il8
  • IL8
  • si:dkey-151b16.2
  • cxcli2
  • cxcl8
  • gcp-1
  • il-8
  • lynap
  • mdncf
  • monap
  • nap-1
  • C-X-C motif chemokine ligand 8
  • interleukin 8
  • chemokine (C-X-C motif) ligand 8a
  • chemokine (C-X-C motif) ligand 1-like
  • chemokine (C-X-C motif) ligand 8 L homeolog
  • CXCL8
  • il8
  • cxcl8a
  • IL8
  • Cxcl8
  • LOC422654
  • cxcl8.L
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
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Detection Method
qPCR
Method Type
Sandwich ELISA
Detection Range
0.038 pg/mL - 600 pg/mL
Minimum Detection Limit
0.038 pg/mL
Application
ELISA
Options
Purpose Human Immunoquantitative (PCR-Based) IL-8/CXCL8 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Semi-Quantitative
Detection Method qPCR
Sensitivity 0.1 pg/mL
Characteristics IQELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
Components
  • IL-8 Microplate (Item A): 96 well PCR plate coated with anti-Human IL-8
  • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
  • Standards (Item C): 2 vials of recombinant Human IL-8
  • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
  • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
  • Detection Affinity Reagent for IL-8 (Item F): 2 vials of a 4x concentrated solution of anti-Human IL-8 affinity reagent
  • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
  • Primer Solution (Item I): 1.7 ml vial
  • PCR Master Mix (Item J): 1.2 ml vial
  • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
  • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
Material not included
  • Real-time PCR instrument, Bio-Rad recommended
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water
  • Log-log graph paper or computer and software for data analysis
  • Tubes to prepare standard or sample dilutions
  • Heating block or water bath capable of 80°C
Plasmids, Primers & others Plasmids, Primers & others Interleukin 8 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name IL-8/CXCL8 (IL8 ELISA Kit Abstract)
Gene ID 3576
UniProt P10145
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Application Notes The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
Comment

Features:
- Excellent for follow up/confirmation of antibody array data
- Pre-coated 96 well plate
- Strip plate format minimizes waste
- Complete kit includes all necessary reagents
- 10x more sensitive than ELISA
- Reproducible and reliable
- Optimized for serum, plasma, urine and cell culture medium
- 1/10th the sample volume of an ELISA

Research Applications:
- Detection of quantitative protein levels in biological fluids
- Validation of antibody array results
- Validation of biomarker discovery studies

Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed
  2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
  3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
  4. Add 15μL Primer solution to each well
  5. Add 10μL of PCR Master Mix to each well
  6. Run real-time PCR
Reagent Preparation

Recommended Dilution for serum and plasma samples2-10 fold

Restrictions For Research Use only
Storage -20 °C
Storage Comment May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Expiry Date 6 months
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