TNFRSF13C ELISA Kit

Catalog No. ABIN6720267

Supplier Product No.: koa0642

The Mouse TNFRSF13C ELISA Kit (ABIN6720267) is a Colorimetric ELISA Kit designed to quantify Mouse TNFRSF13C.

Quick Overview for TNFRSF13C ELISA Kit (ABIN6720267)

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details TNFRSF13C ELISA Kit

Minimum Detection Limit: 78 pg/mL

Supplier Product No.: koa0642

Supplier: Rockland

Purpose: Mouse TNFRSF13C - BAFFR Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse TNFRSF13C. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse TNFRSF13C standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFRSF13C standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: S10-A71

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TNFRSF13C

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Alternative Name: TNFRSF13C/BAFFR

Background:

Synonyms: B Cell Activating Factor Receptor, B-cell-activating factor receptor, BAFF R, BAFF receptor, BAFF-R, BAFFR, BLyS receptor 3, BlySR3, BR 3, BR3, BROMIX, CD 268, CD268, CD268 antigen, CVID4, MGC138235, OTTHUMP00000028746, Prolixin, TNFRSF 13C, TNFRSF13C, TR13C_HUMAN, Tumor Necrosis Factor Receptor Subunit Member 13C, Tumor necrosis factor receptor superfamily member 13C

Background: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Gene ID: 72049

NCBI Accession: NP_082351

UniProt: Q9D8D0

Pathways: NF-kappaB Signaling