TNFRSF13C ELISA Kit

Catalog No. ABIN6951091

Product Details TNFRSF13C ELISA Kit

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human BAFF R ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for TNFRSF13C

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Alternative Name: BAFF R (TNFRSF13C Products)

Synonyms: RGD1560810 ELISA Kit, TNFRSF13C ELISA Kit, BAFF-R ELISA Kit, BAFFR ELISA Kit, BROMIX ELISA Kit, CD268 ELISA Kit, CVID4 ELISA Kit, prolixin ELISA Kit, 2010006P15Rik ELISA Kit, Baffr ELISA Kit, Bcmd ELISA Kit, Bcmd-1 ELISA Kit, Bcmd1 ELISA Kit, Lvis22 ELISA Kit, TNF receptor superfamily member 13C ELISA Kit, uncharacterized LOC746611 ELISA Kit, tumor necrosis factor receptor superfamily, member 13c ELISA Kit, TNFRSF13C ELISA Kit, Tnfrsf13c ELISA Kit, LOC746611 ELISA Kit

Gene ID: 115650

UniProt: Q96RJ3

Pathways: NF-kappaB Signaling