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TNFRSF10B ELISA Kit
TNFRSF10B
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details TNFRSF10B ELISA Kit
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Target
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TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
Reactivity
All reactivities for TNFRSF10B elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse TRAIL R2/TNFRSF10B ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse TRAIL R2.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TNFRSF10B
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Target
See all TNFRSF10B ELISA Kits
TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
Alternative Name
TRAIL R2 (TNFRSF10B (TNFRSF10B Products )
Synonyms
TNFRSF10B ELISA Kit, dr5 ELISA Kit, cd262 ELISA Kit, killer ELISA Kit, trick2 ELISA Kit, trickb ELISA Kit, xDR-M1 ELISA Kit, xDR-M2 ELISA Kit, ztnfr9 ELISA Kit, trailr2 ELISA Kit, trick2a ELISA Kit, trick2b ELISA Kit, CD262 ELISA Kit, DR5 ELISA Kit, KILLER ELISA Kit, KILLER/DR5 ELISA Kit, TRAIL-R2 ELISA Kit, TRAILR2 ELISA Kit, TRICK2 ELISA Kit, TRICK2A ELISA Kit, TRICK2B ELISA Kit, TRICKB ELISA Kit, ZTNFR9 ELISA Kit, Ly98 ELISA Kit, MK ELISA Kit, tumor necrosis factor receptor superfamily, member 10b ELISA Kit, tumor necrosis factor receptor superfamily member 10b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b L homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b ELISA Kit, TNF receptor superfamily member 10b ELISA Kit, Tnfrsf10b ELISA Kit, TNFRSF10B ELISA Kit, tnfrsf10b.S ELISA Kit, tnfrsf10b.L ELISA Kit, tnfrsf10b ELISA Kit
UniProt
Q9QZM4
Pathways
p53 Signaling , Apoptosis , Positive Regulation of Endopeptidase Activity
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