CDK1 ELISA Kit (Cyclin-Dependent Kinase 1)

Details for Product CDK1 ELISA Kit No. ABIN6730453
Target
Binding Specificity
pThr161
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
5
5
3
2
1
1
1
1
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human Phospho-CDK1 (Thr161) and Total CDK1 ELISA Kit. This assay semi-quantitatively measures CDK1 phosphorylated at Threonine-161 as well as total CDK1 in cell lysate samples.
Sample Type Cell Samples, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human CDK1 phosphorylated at site Threonine-161 as well as total CDK1.
Characteristics
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Target
Alternative Name CDK1 (CDK1 ELISA Kit Abstract)
Gene ID 983
UniProt P06493
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Images
Image no. 1 for Cyclin-Dependent Kinase 1 (CDK1) ELISA Kit (ABIN6730453) HeLa cells were treated or untreated with Hydroxyurea. Cell lysates were analyzed usi...
Image no. 2 for Cyclin-Dependent Kinase 1 (CDK1) ELISA Kit (ABIN6730453) HeLa cells were treated with Hydroxyurea. Solubilize cells at 4 x 10^7 cells/ml in Ce...
Image no. 3 for Cyclin-Dependent Kinase 1 (CDK1) ELISA Kit (ABIN6730453) HeLa cells were treated or untreated with Hydroxyurea. Cell lysates were analyzed usi...