WNT4 ELISA Kit

Catalog No. ABIN6730511

Product Details WNT4 ELISA Kit

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Wnt-4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Wnt-4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for WNT4

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Alternative Name: Wnt-4 (WNT4 Products)

Synonyms: CG4698 ELISA Kit, DWnt-4 ELISA Kit, DWnt4 ELISA Kit, Dm DWnt4 ELISA Kit, Dmel\\CG4698 ELISA Kit, Dwnt4 ELISA Kit, Wnt ELISA Kit, Wnt-4 ELISA Kit, anon-EST:Liang-2.4 ELISA Kit, clone 2.4 ELISA Kit, wnt-4 ELISA Kit, wnt4 ELISA Kit, SERKAL ELISA Kit, WNT-4 ELISA Kit, Xwnt4 ELISA Kit, xwnt-4 ELISA Kit, zgc:136737 ELISA Kit, Wnt family member 4 ELISA Kit, Wnt oncogene analog 4 ELISA Kit, wingless-type MMTV integration site family, member 4 ELISA Kit, wingless-type MMTV integration site family member 4 S homeolog ELISA Kit, wingless-type MMTV integration site family, member 4a ELISA Kit, WNT4 ELISA Kit, Wnt4 ELISA Kit, wnt4.S ELISA Kit, wnt4a ELISA Kit

UniProt: P22724

Pathways: WNT Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation