PRKAR1A ELISA Kit

Catalog No. ABIN6730540

Product Details PRKAR1A ELISA Kit

Target: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PRKAR1A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human PRKAR1A.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PRKAR1A

Target: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Alternative Name: PRKAR1A (PRKAR1A Products)

Synonyms: ACRDYS1 ELISA Kit, ADOHR ELISA Kit, CAR ELISA Kit, CNC ELISA Kit, CNC1 ELISA Kit, PKR1 ELISA Kit, PPNAD1 ELISA Kit, PRKAR1 ELISA Kit, TSE1 ELISA Kit, 1300018C22Rik ELISA Kit, RIalpha ELISA Kit, Tse-1 ELISA Kit, Tse1 ELISA Kit, RIIA ELISA Kit, prkar1a ELISA Kit, zgc:92515 ELISA Kit, im:7047729 ELISA Kit, cnc1 ELISA Kit, pkr1 ELISA Kit, ppnad1 ELISA Kit, prkar1 ELISA Kit, tse1 ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha ELISA Kit, protein kinase, cAMP dependent regulatory, type I, alpha ELISA Kit, protein kinase cAMP-dependent type 1 regulatory subunit alpha ELISA Kit, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a ELISA Kit, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog ELISA Kit, PRKAR1A ELISA Kit, Prkar1a ELISA Kit, prkar1aa ELISA Kit, LOC707275 ELISA Kit, prkar1a.S ELISA Kit

UniProt: P10644

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma