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MERTK ELISA Kit (C-Mer Proto-Oncogene Tyrosine Kinase) ELISA Kit

MERTK Reactivity: Human AA 26-499 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Serum, Tissue Homogenate
Catalog No. ABIN921101
$430.00
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96 tests
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  • Target
    MERTK
    Binding Specificity
    AA 26-499
    Reactivity
    • 5
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Mer
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: sf21
    Immunogen sequence: R26-A499
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: sf21
    Immunogen sequence: R26-A499
    Featured
    Discover our best selling MERTK ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

    Tissue Specificity: Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

    Plate
    Pre-coated
    Protocol
    human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Mer has been precoated onto 96-well plates. Standards(sf21, R26-A499) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Mer is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mer amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Mer standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Mer standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1124, Standard deviation: 58.45, CV(%): 5.2
    • Sample 2: n=16, Mean(pg/ml): 3287, Standard deviation: 210.4, CV(%): 6.4
    • Sample 3: n=16, Mean(pg/ml): 5853, Standard deviation: 280.9, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 1374, Standard deviation: 92.1, CV(%): 6.7
    • Sample 2: n=24, Mean(pg/ml): 3467, Standard deviation: 249.6, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 6124, Standard deviation: 306.2, CV(%): 5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target
    MERTK
    Alternative Name
    MERTK (MERTK ELISA Kit Abstract)
    Synonyms
    MER, RP38, c-mer, Eyk, Mer, Nyk, nmf12, rdy, C-EYK, MERTK, DKFZp469D2215, si:ch1073-268l12.2, MER proto-oncogene, tyrosine kinase, c-mer proto-oncogene tyrosine kinase, c-mer proto-oncogene tyrosine kinase a, MERTK, Mertk, mertka
    Background

    Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

    Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium(RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa(RP)

    Synonyms: Tyrosine-protein kinase Mer,2.7.10.1,Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,MERTK,MER,

    Full Gene Name: Tyrosine-protein kinase Mer

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    10461
    UniProt
    Q12866
    Pathways
    RTK Signaling
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