V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Peptide
AKT1
Host: Synthetic
WB
Without preservative
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- Target See all AKT1 products
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- Peptide Type
- Synthetic
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Source
- Synthetic
- Application
- Western Blotting (WB)
- Supplier Product No.
- 000-000-260
- Supplier
- Rockland
- Purpose
- Substrate Peptide AKT/PKBa
- Characteristics
- AKT1, serine/threonine kinase, apoptosis inhibitor substrate peptides, blocking peptide, control peptide
- Purity
- Greater than 98% specific peptide by HPLC analysis
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- Application Notes
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Application Note: AKT/PKBa Substrate control peptide should be used at 1.0 μg per 1.0 μL of antiserum in per assay.
Western Blot Dilution: 1:500 - 1:2,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 500 μL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: None
Stabilizer: None
Preservative: None - Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- Background
- Background: Akt is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3) at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
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