Ligase IV, DNA, ATP-Dependent (LIG4) Peptide
LIG4
Reactivity: Mammalian
Host: Synthetic
BP, IHC, WB
Catalog No. ABIN937334
Quick Overview for Ligase IV, DNA, ATP-Dependent (LIG4) Peptide (ABIN937334)
Target
LIG4
(Ligase IV, DNA, ATP-Dependent (LIG4))
Origin
Mammalian
Source
Synthetic
Application
Blocking Peptide (BP), Immunohistochemistry (IHC), Western Blotting (WB)
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Peptide Type
- Synthetic
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Sequence
- DGERMQMHKD GDVYKYFSRN GYNYTDQFGA SPTEGSLTPF IHNAFKADIQ
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Characteristics
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A synthetic peptide for use as a blocking control in assays to test for specificity of LIG4 antibody,
Alternative Names: LIG4 control peptide, LIG4 antibody Blocking Peptide, Anti-LIG4 Blocking Peptide, Ligase Iv Dna Atp-Dependent Blocking Peptide, LIG4, LIG-4, LIG 4, LIG-4 Blocking Peptide, LIG 4 Blocking Peptide
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Application Notes
- Optimal conditions should be determined by the investigator
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 µL of distilled water for a final peptide concentration is 1 mg/mL.
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Buffer
- PBS
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C long term.
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- LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))
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Background
- LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
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Molecular Weight
- 104 kDa
Target
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