anti-Mouse (Murine) CD74 antibody for Immunohistochemistry

Recommended CD74 Antibody

Antigen
HLA-DR-gamma (CD74) Antibodies
  • DHLAG
  • HLADG
  • II
  • Ia-GAMMA
  • CLIP
  • Ii
  • INVG34
  • Iclp-1
  • iclp1
  • wu:fb11f09
  • wu:fb12a01
  • wu:fb13d06
  • wu:fk94e07
  • CD74 molecule
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain a
  • CD74
  • Cd74
  • cd74a
Reactivity
Human, Mouse (Murine)
534
120
31
29
Host
Mouse
452
105
10
2
Clonality
Monoclonal
Conjugate
This CD74 antibody is conjugated to Atto 655
36
32
25
20
19
16
14
9
9
9
9
9
9
9
9
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
347
339
233
135
98
95
64
63
60
58
16
13
10
2
2
2
2
2
1
1
Options

Available images

Catalog No. ABIN2481703
$ 439.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
27.251339 ABIN2481704 ICC FACS IF IP IHC WB Atto 680 Mouse IgG1 PIN-1 1
27.251339 ABIN2481700 ICC FACS IF IP IHC WB Atto 565 Mouse IgG1 PIN-1 1
27.251339 ABIN2481707 ICC FACS IF IP IHC WB APC Mouse IgG1 PIN-1 1
27.251339 ABIN2481709 ICC FACS IF IP IHC WB FITC Mouse IgG1 PIN-1 1
27.251339 ABIN2481698 ICC FACS IF IP IHC WB Atto 390 Mouse IgG1 PIN-1 1
27.251339 ABIN2481701 ICC FACS IF IP IHC WB Atto 594 Mouse IgG1 PIN-1 1
27.251339 ABIN2481705 ICC FACS IF IP IHC WB Atto 700 Mouse IgG1 PIN-1 1
27.251339 ABIN2481699 ICC FACS IF IP IHC WB Atto 488 Mouse IgG1 PIN-1 1
27.251339 ABIN2481711 ICC FACS IF IP IHC WB PE-Atto 594 Mouse IgG1 PIN-1 1
27.251339 ABIN2481712 ICC FACS IF IP IHC WB PerCP Mouse IgG1 PIN-1 1
27.251339 ABIN2481702 ICC FACS IF IP IHC WB Atto 633 Mouse IgG1 PIN-1 1
27.251339 ABIN2481713 ICC FACS IF IP IHC WB PE Mouse IgG1 PIN-1 1
27.251339 ABIN2481706 ICC FACS IF IP IHC WB Alkaline Phosphatase (AP) Mouse IgG1 PIN-1 1
27.251339 ABIN2481708 ICC FACS IF IP IHC WB Biotin Mouse IgG1 PIN-1 1
27.251339 ABIN2481710 ICC FACS IF IP IHC WB HRP Mouse IgG1 PIN-1 1
27.251339 ABIN2481714 ICC FACS IF IP IHC WB Streptavidin Mouse IgG1 PIN-1 1
19.70443 ABIN361789 ICC FACS IF IP IHC WB Mouse IgG1 PIN-1 8
12.204429 ABIN6941259 FACS IHC StM WB Rabbit IgG CLIP-3127R 0
12.204429 ABIN6931005 FACS ICC IF IHC IP WB Cy7,APC Mouse IgG1 PIN-1 0
12.204429 ABIN6931006 FACS ICC IF IHC IP WB DyLight 350 Mouse IgG1 PIN-1 0

General

Antigen HLA-DR-gamma (CD74) Antibodies
Reactivity Human, Mouse (Murine)
(534), (120), (31), (29)
Host Mouse
(452), (105), (10), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD74 antibody is conjugated to Atto 655
(36), (32), (25), (20), (19), (16), (14), (9), (9), (9), (9), (9), (9), (9), (9), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(347), (339), (233), (135), (98), (95), (64), (63), (60), (58), (16), (13), (10), (2), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-CD74 Antibody

Target Details CD74 Application Details Handling References for anti-CD74 antibody (ABIN2481703) Images
Specificity Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Purification Protein G Purified
Immunogen Human CD74 invariant chain synthetic peptide
Clone PIN-1
Isotype IgG1
Plasmids, Primers & others

Target Details CD74

Product Details anti-CD74 Antibody Application Details Handling References for anti-CD74 antibody (ABIN2481703) Images back to top
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Gene ID 972
NCBI Accession NP_001020329
UniProt P04233
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints

Application Details

Product Details anti-CD74 Antibody Target Details CD74 Handling References for anti-CD74 antibody (ABIN2481703) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-116 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-CD74 Antibody Target Details CD74 Application Details References for anti-CD74 antibody (ABIN2481703) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-CD74 antibody (ABIN2481703)

Product Details anti-CD74 Antibody Target Details CD74 Application Details Handling Images back to top
Background publications

Denzin, Robbins, Carboy-Newcomb, Cresswell: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995

Images

Product Details anti-CD74 Antibody Target Details CD74 Application Details Handling References for anti-CD74 antibody (ABIN2481703) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-HLA-DR-gamma (CD74) antibody (Atto 655) (ABIN2481703) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Anti...
Immunohistochemistry (IHC) image for anti-HLA-DR-gamma (CD74) antibody (Atto 655) (ABIN2481703) Immunohistochemistry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1....
Western Blotting (WB) image for anti-HLA-DR-gamma (CD74) antibody (Atto 655) (ABIN2481703) Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein usi...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-HLA-DR-gamma (CD74) antibody (Atto 655) (ABIN2481703) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Anti...