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CD74 antibody (PE)

CD74 Reactivity: Human WB, FACS, IF, IHC, IP, ICC Host: Mouse Monoclonal PIN-1 PE
Catalog No. ABIN2481713
  • Target See all CD74 Antibodies
    CD74 (HLA-DR-gamma (CD74))
    Reactivity
    • 182
    • 64
    • 13
    • 11
    Human
    Host
    • 120
    • 80
    • 3
    • 2
    Mouse
    Clonality
    • 136
    • 67
    • 2
    Monoclonal
    Conjugate
    • 69
    • 17
    • 16
    • 10
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This CD74 antibody is conjugated to PE
    Application
    • 125
    • 109
    • 78
    • 44
    • 36
    • 36
    • 28
    • 26
    • 18
    • 14
    • 13
    • 11
    • 9
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
    Cross-Reactivity
    Human, Mouse
    Purification
    Protein G Purified
    Immunogen
    Human CD74 invariant chain synthetic peptide
    Clone
    PIN-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD74 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2481713 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    CD74 (HLA-DR-gamma (CD74))
    Alternative Name
    CD74 (CD74 Products)
    Synonyms
    DHLAG antibody, HLADG antibody, II antibody, Ia-GAMMA antibody, CLIP antibody, Ii antibody, INVG34 antibody, Iclp-1 antibody, iclp1 antibody, wu:fb11f09 antibody, wu:fb12a01 antibody, wu:fb13d06 antibody, wu:fk94e07 antibody, Iclp-2 antibody, cd74 antibody, iclp2 antibody, zgc:123161 antibody, CD74 molecule antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 molecule, major histocompatibility complex, class II invariant chain a antibody, CD74 molecule, major histocompatibility complex, class II invariant chain b antibody, CD74 antibody, Cd74 antibody, cd74a antibody, cd74b antibody
    Background
    CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
    Gene ID
    972
    NCBI Accession
    NP_001020329
    UniProt
    P04233
    Pathways
    Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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