ANAPC5 Protein (AA 2-232, partial) (GST tag)

Catalog No. ABIN1046520

Product Details

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Protein Type: Recombinant

Protein Characteristics: AA 2-232, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ANAPC5 protein is labelled with GST tag.

Application: SDS-PAGE (SDS), ELISA

Sequence: ASVHESLYFN PMMTNGVVHA NVFGIKDWVT PYKIAVLVLL NEMSRTGEGA VSLMERRRLN QLLLPLLQGP DITLSKLYKL IEESCPQLAN SVQIRIKLMA EGELKDMEQF FDDLSDSFSG TEPEVHKTSV VGLFLRHMIL AYSKLSFSQV FKLYTALQQY FQNGEKKTVE DADMELTSRD EGERKMEKEE LDVSVREEEV SCSGPLSQKQ AEFFLSQQAS LLKNDETKAL T

Characteristics: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Ref.7

Purity: 90 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS buffer, 20mM GSH

Storage: -20 °C

References

Bechtel, Rosenfelder, Duda, Schmidt, Ernst, Wellenreuther, Mehrle, Schuster, Bahr, Blöcker, Heubner, Hoerlein, Michel, Wedler, Köhrer, Ottenwälder, Poustka, Wiemann, Schupp: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, (2008) (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, (2004) (PubMed).

Beausoleil, Jedrychowski, Schwartz, Elias, Villén, Li, Cohn, Cantley, Gygi: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, (2004) (PubMed).

Kraft, Herzog, Gieffers, Mechtler, Hagting, Pines, Peters: "Mitotic regulation of the human anaphase-promoting complex by phosphorylation." in: The EMBO journal, Vol. 22, Issue 24, pp. 6598-609, (2003) (PubMed).

Yu, Peters, King, Page, Hieter, Kirschner: "Identification of a cullin homology region in a subunit of the anaphase-promoting complex." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1219-22, (1998) (PubMed).

Target Details

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Alternative Name: Anaphase-promoting complex subunit 5 (ANAPC5 Products)

Synonyms: APC5 Protein, DDBDRAFT_0206386 Protein, DDBDRAFT_0234269 Protein, DDB_0206386 Protein, DDB_0234269 Protein, zgc:55800 Protein, wu:fb75a09 Protein, ANAPC5 Protein, MGC89697 Protein, 2510006G12Rik Protein, AA408751 Protein, AA536819 Protein, AA986414 Protein, Anpc5 Protein, F12K11.7 Protein, F12K11_7 Protein, anaphase promoting complex subunit 5 Protein, anaphase-promoting complex subunit 5 Protein, anaphase promoting complex subunit 5 L homeolog Protein, anaphase-promoting complex subunit Protein, anaphase-promoting complex subunit Apc5 Protein, ANAPC5 Protein, anapc5 Protein, LOC100093215 Protein, anapc5.L Protein, Anapc5 Protein, AT1G06590 Protein, apc5 Protein

Background: Synonyms: APC5,Cyclosome subunit 5

Molecular Weight: 53.6 kD

UniProt: Q9UJX4

Pathways: M Phase