CCL14 Protein (AA 25-82, partial) (GST tag)

Catalog No. ABIN1046663

Product Details

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Protein Type: Recombinant

Protein Characteristics: AA 25-82, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CCL14 protein is labelled with GST tag.

Application: ELISA

Sequence: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVP

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Nomiyama, Fukuda, Iio, Tanase, Miura, Yoshie: "Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 19, Issue 3, pp. 227-34, (1999) (PubMed).

Pardigol, Forssmann, Zucht, Loetscher, Schulz-Knappe, Baggiolini, Forssmann, Mägert: "HCC-2, a human chemokine: gene structure, expression pattern, and biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6308-13, (1998) (PubMed).

Schulz-Knappe, Mägert, Dewald, Meyer, Cetin, Kubbies, Tomeczkowski, Kirchhoff, Raida, Adermann, Kist, Reinecke, Sillard, Pardigol, Uguccioni, Baggiolini, Forssmann: "HCC-1, a novel chemokine from human plasma." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 295-9, (1996) (PubMed).

Target Details

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Alternative Name: C-C motif chemokine 14 protein (CCL14 Products)

Synonyms: CCL14 Protein, CC-1 Protein, CC-3 Protein, CKB1 Protein, HCC-1 Protein, HCC-1(1-74) Protein, HCC-1/HCC-3 Protein, HCC-3 Protein, MCIF Protein, NCC-2 Protein, NCC2 Protein, SCYA14 Protein, SCYL2 Protein, SY14 Protein, C-C motif chemokine ligand 14 Protein, C-C motif chemokine 14 Protein, chemokine (C-C motif) ligand 14 Protein, CCL14 Protein, Ccl14 Protein, LOC480603 Protein, LOC101115044 Protein

Background: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.

Molecular Weight: 34.1 kD

UniProt: O00626