CXCL1 Protein (AA 35-107, full length) (His tag)

Catalog No. ABIN1047116

Product Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Protein Type: Recombinant

Protein Characteristics: AA 35-107, full length

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CXCL1 protein is labelled with His tag.

Application: ELISA

Sequence: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Baker, Kucera, Richmond: "Nucleotide sequence of the human melanoma growth stimulatory activity (MGSA) gene." in: Nucleic acids research, Vol. 18, Issue 21, pp. 6453, (1991) (PubMed).

Richmond, Balentien, Thomas, Flaggs, Barton, Spiess, Bordoni, Francke, Derynck: "Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin." in: The EMBO journal, Vol. 7, Issue 7, pp. 2025-33, (1988) (PubMed).

Anisowicz, Bardwell, Sager: "Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 20, pp. 7188-92, (1987) (PubMed).

Target Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: Growth-Regulated alpha Protein (CXCL1 Products)

Synonyms: FSP Protein, GRO1 Protein, GROa Protein, MGSA Protein, MGSA-a Protein, NAP-3 Protein, SCYB1 Protein, CINC-1 Protein, Gro1 Protein, CXCL1 Protein, Sdf1 Protein, Fsp Protein, KC Protein, Mgsa Protein, N51 Protein, Scyb1 Protein, gro Protein, GRO Protein, Gro Protein, C-X-C motif chemokine ligand 1 Protein, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) Protein, C-X-C motif chemokine ligand 12 Protein, chemokine (C-X-C motif) ligand 1 Protein, CXCL1 Protein, Cxcl1 Protein, Cxcl12 Protein, GRO1 Protein

Background: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Molecular Weight: 12 kD

UniProt: P09341

Pathways: Autophagy