CXCL1 Protein

Catalog No. ABIN6699662

Supplier Product No.: 009-001-v14-0005

Product Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-v14-0005

Supplier: Rockland

Purpose: Human Gro Alpha (CXCL1) Recombinant Protein

Purification: Gro Alpha (CXCL1) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human neutrophils at concentrations between 10-100 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Gro Alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Gro Alpha in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1 (CXCL1 Products)

Background:

Synonyms: C-X-C motif chemokine 1, GRO-alpha(1-73) MGSAα, mKC, NAP-3, GRO1, rCINC

Background: Growth Regulated Protein alpha (GROα), also known as CXCL1, is a chemokine thought to have mitogenic properties and chemoattract neutrophils. Secreted by macrophages, epithelial cells, neutrophils and melanomas, GROα signals through chemokine receptor, CXCR2, and has been implicated in the processes of spinal cord formation, inflammation, angiogenesis, tumorigenesis, and wound healing. Recombinant human GROα is a non-glycosylated protein, containing 73 amino acids, with a molecular weight of 7.9 kDa.

UniProt: P09341

Pathways: Autophagy