beta Actin Protein (AA 2-375) (His tag)

Catalog No. ABIN1096107

Product Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Protein Type: Recombinant

Protein Characteristics: AA 2-375

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This beta Actin protein is labelled with His tag.

Purpose: Recombinant Human β-Actin/ACTB (C-6His)

Sequence: DDDIAALVVD NGSGMCKAGF AGDDAPRAVF PSIVGRPRHQ GVMVGMGQKD SYVGDEAQSK RGILTLKYPI EHGIVTNWDD MEKIWHHTFY NELRVAPEEH PVLLTEAPLN PKANREKMTQ IMFETFNTPA MYVAIQAVLS LYASGRTTGI VMDSGDGVTH TVPIYEGYAL PHAILRLDLA GRDLTDYLMK ILTERGYSFT TTAEREIVRD IKEKLCYVAL DFEQEMATAA SSSSLEKSYE LPDGQVITIG NERFRCPEAL FQPSFLGMES CGIHETTFNS IMKCDVDIRK DLYANTVLSG GTTMYPGIAD RMQKEITALA PSTMKIKIIA PPERKYSVWI GGSILASLST FQQMWISKQE YDESGPSIVH RKCFLEHHHH HH

Characteristics: Recombinant Human beta-Actin/ACTB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Phe375) of Human ACTB fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 10 mM TrisHCl, 0.1 % TritonX-100, 2 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin (ACTB Products)

Synonyms: BRWS1 Protein, PS1TP5BP1 Protein, ACTA1 Protein, Actx Protein, E430023M04Rik Protein, beta-actin Protein, ps1tp5bp1 Protein, act-2 Protein, actb Protein, B-actin Protein, LOC100174883 Protein, LOC100304816 Protein, A Protein, ACT5C Protein, Ac5C Protein, Act Protein, Act-5C Protein, Act5 Protein, Act5c Protein, Actin Protein, Actin/BAP47 Protein, Actin5C Protein, BAP47 Protein, Bap47 Protein, CG4027 Protein, Dmel\\CG4027 Protein, M32055 Protein, T11 Protein, act Protein, act 5C Protein, act5C Protein, actin Protein, actin5C Protein, anon-EST:fe2D2 Protein, beta-actin/Bap47 Protein, cyt5C Protein, l(1)G0009 Protein, l(1)G0010 Protein, l(1)G0025 Protein, l(1)G0079 Protein, l(1)G0117 Protein, l(1)G0177 Protein, l(1)G0245 Protein, l(1)G0330 Protein, l(1)G0420 Protein, l(1)G0486 Protein, Bact Protein, ACT-5 Protein, ACTB Protein, B-ACTZF Protein, actba Protein, bact Protein, bactin1 Protein, bactzf Protein, wu:fd18f05 Protein, A26C1B Protein, POTE2alpha Protein, POTEACTIN Protein, Beta-actin Protein, actin beta Protein, actin, beta Protein, cytoplasmic actin OlCA1 Protein, beta-actin Protein, Actin 5C Protein, actin gamma 1 Protein, actin, beta 1 Protein, actin, beta L homeolog Protein, beta actin Protein, POTE ankyrin domain family member F Protein, actin, beta like 2 Protein, actin, cytoplasmic 1 Protein, ACTB Protein, Actb Protein, actb Protein, LOC100174883 Protein, LOC100304816 Protein, Act5C Protein, ACTG1 Protein, actb1 Protein, actb.L Protein, LOC100136352 Protein, POTEF Protein, ACTBL2 Protein, LOC109073280 Protein

Sub Type: Fusionprotein

Background: Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Alternative Names: Actin Cytoplasmic 1, Beta-Actin, ACTB

Molecular Weight: 42.8 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction