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Actin, beta (ACTB) (AA 2-375) protein (His tag) Protein

ACTB Origin: Human Source: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1096107
$522.50
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  • Protein Name
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-375
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This beta Actin protein is labelled with His tag.
    Purpose
    Recombinant Human β-Actin/ACTB (C-6His)
    Sequence
    DDDIAALVVD NGSGMCKAGF AGDDAPRAVF PSIVGRPRHQ GVMVGMGQKD SYVGDEAQSK RGILTLKYPI EHGIVTNWDD MEKIWHHTFY NELRVAPEEH PVLLTEAPLN PKANREKMTQ IMFETFNTPA MYVAIQAVLS LYASGRTTGI VMDSGDGVTH TVPIYEGYAL PHAILRLDLA GRDLTDYLMK ILTERGYSFT TTAEREIVRD IKEKLCYVAL DFEQEMATAA SSSSLEKSYE LPDGQVITIG NERFRCPEAL FQPSFLGMES CGIHETTFNS IMKCDVDIRK DLYANTVLSG GTTMYPGIAD RMQKEITALA PSTMKIKIIA PPERKYSVWI GGSILASLST FQQMWISKQE YDESGPSIVH RKCFLEHHHH HH
    Characteristics
    Recombinant Human beta-Actin/ACTB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Phe375) of Human ACTB fused with a 6His tag at the C-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Supplied as a 0.2 μm filtered solution of 10 mM TrisHCl, 0.1 % TritonX-100, 2 mM DTT, pH 8.0.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    -80 °C
    Storage Comment
    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Expiry Date
    6 months
  • Protein Name
    Sub Type
    Fusionprotein
    Background
    Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
    Alternative Names: Actin Cytoplasmic 1, Beta-Actin, ACTB
    Molecular Weight
    42.8 kDa
    UniProt
    P60709
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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