beta Actin Protein (AA 1-375) (His tag)
Quick Overview for beta Actin Protein (AA 1-375) (His tag) (ABIN7505455)
Target
See all beta Actin (ACTB) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-375
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Purification tag / Conjugate
- This beta Actin protein is labelled with His tag.
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Sequence
- Met1-Phe375
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Characteristics
- A DNA sequence encoding the Danio rerio Beta-actin/ACTB (Q7ZVF9) (1M-375F) was expressed with a polyhistidine tag at the N-terminus.
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Actin, beta (ACTB) (AA 1-375) protein (His tag)
custom-made ACTB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (His tag)custom-made ACTB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (GST tag)ACTB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Actin, beta (ACTB) (AA 14-167) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Actin, beta (ACTB) (AA 102-375) protein (His-IF2DI Tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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used as Immunogen for (ABIN6999909, ABIN7234259, ABIN7234260 and ABIN7234261)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- beta Actin (ACTB) (Actin, beta (ACTB))
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Alternative Name
- Beta-actin
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Background
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Abbreviation: Beta-actin,ACTB
Target Synonym: A26C1A,A26C1B,ACTBB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1
Background: Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA
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Molecular Weight
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Calculated MW: 43.1 kDa
Observed MW: 42-48 kDa
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UniProt
- Q7ZVF9
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Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Target
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