IPP Isomerase 2 Protein (IDI2) (AA 1-227) (His tag)
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- Target See all IPP Isomerase 2 (IDI2) Proteins
- IPP Isomerase 2 (IDI2)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-227
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IPP Isomerase 2 protein is labelled with His tag.
- Purpose
- Recombinant Human Isopentenyl Pyrophosphate Isomerase 2//IPPI2/IDI2 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSDINLDWVD RRQLQRLEEM LIVVDENDKV IGADTKRNCH LNENIEKGLL HRAFSVVLFN TKNRILIQQR SDTKVTFPGY FTDSCSSHPL YNPAELEEKD AIGVRRAAQR RLQAELGIPG EQISPEDIVF MTIYHHKAKS DRIWGEHEIC YLLLVRKNVT LNPDPSETKS ILYLSQEELW ELLEREARGE VKVTPWLRTI AERFLYRWWP HLDDVTPFVE LHKIHRV
- Characteristics
- Recombinant Human Isopentenyl Pyrophosphate Isomerase 2/IPP Isomerase 2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val227) of Human IDI2 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product IDI2 Protein
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IPP Isomerase 2 (IDI2) (AA 2-227) protein (His tag)
IDI2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
IPP Isomerase 2 (IDI2) (AA 1-227) protein (GST tag)IDI2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
IPP Isomerase 2 (IDI2) protein (Myc-DYKDDDDK Tag)IDI2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 0.1 mM PMSF, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- IPP Isomerase 2 (IDI2)
- Alternative Name
- IPP-isomerase-2 (IDI2 Products)
- Sub Type
- Fusionprotein
- Background
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Isopentenyl Pyrophosphate Isomerase 2 (IDI2) belongs to the IPP isomerase type 1 family. Both isozymes, IDI1 and IDI2 are localized to the peroxisome by a PTS1-dependent pathway. IDI2 is expressed in skeletal muscle, which contains one nudix hydrolase domain. IDI2 binds one magnesium per subunit. IDI2 catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). It is reported that IDI2 is regulated independently from IDI1, by a mechanism that may involve PPAR-alpha.
Alternative Names: Isopentenyl-Diphosphate Delta-Isomerase 2, Isopentenyl Pyrophosphate Isomerase 2, IPP Isomerase 2, IPPI2, IDI2 - Molecular Weight
- 28.9 kDa
- UniProt
- Q9BXS1
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