SENP7 Protein (AA 695-864) (His tag)

Catalog No. ABIN1097579

Product Details

Target: SENP7 (SUMO1/sentrin Specific Protease 7 (SENP7))

Protein Type: Recombinant

Protein Characteristics: AA 695-864

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SENP7 protein is labelled with His tag.

Purpose: Recombinant Human Sentrin-Specific Protease 7/SENP7 (N-6His)

Sequence: MKLKSVSQPS NTDAAKPTYT FLQKQSSGCY SLSITSNPDE EWREVRHTGL VQKLIVYPPP PTKGGLGVTN EDLECLEEGE FLNDVIIDFY LKYLILEKAS DELVERSHIF SSFFYKCLTR KENNLTEDNP NLSMAQRRHK RVRTWTRHIN IFNKDYIFVP VNESSHWYLA

Characteristics: Recombinant Human Sentrin-Specific Protease 7/SENP7 is produced by our E. coli expression system. The target protein is expressed with sequence (Met695-Ala864) of Human SENP7.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 5 % Glycerol, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SENP7 (SUMO1/sentrin Specific Protease 7 (SENP7))

Alternative Name: Sentrin-Specific Protease 7/SENP7 (SENP7 Products)

Background: Sentrin-Specific Protease 7 (SENP7) acts as a SUMO-2/3-specific protease. SENP7 is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo. SENP7 has a restricted substrate specificity, and displaying paralogue-specific isopeptidase activity. The C-terminal catalytic domain of SENP7 depolymerized poly-SUMO-2 chains but does not have activity against poly-SUMO-1 chains. SENP7 also had isopeptidase activity against di-SUMO-2- and SUMO-2-modified RanGAP1 (Ran GTPase-activating protein 1) but had limited activity against SUMO-1-modified RanGAP1.
Alternative Names: Sentrin-Specific Protease 7, SUMO-1-Specific Protease 2, Sentrin/SUMO-Specific Protease SENP7, SENP7, KIAA1707, SSP2, SUSP2

Molecular Weight: 27.3 kDa

UniProt: Q9BQF6