KLF4 Protein (AA 1-470) (GST tag)

Catalog No. ABIN1308754

Product Details

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Protein Type: Recombinant

Protein Characteristics: AA 1-470

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This KLF4 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: KLF4 (Human) Recombinant Protein (P01)

Sequence: MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLPPVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPSTCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD INDVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGSCMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRAFSRSD HLALHMKRHF

Characteristics: Human KLF4 full-length ORF ( NP_004226.2, 1 a.a. - 470 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Khan, Narayanan, Lu, Choo, Du, Wiradharma, Yang, Wan: "Delivery of reprogramming factors into fibroblasts for generation of non-genetic induced pluripotent stem cells using a cationic bolaamphiphile as a non-viral vector." in: Biomaterials, Vol. 34, Issue 21, pp. 5336-43, (2013) (PubMed).

Rivero, Díaz-Guerra, Monsalve, Laborda, García-Ramírez: "DLK2 is a transcriptional target of KLF4 in the early stages of adipogenesis." in: Journal of molecular biology, Vol. 417, Issue 1-2, pp. 36-50, (2012) (PubMed).

Target Details

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4 (KLF4 Products)

Background: Full Gene Name: Kruppel-like factor 4 (gut)
Synonyms: EZF,GKLF

Gene ID: 9314

NCBI Accession: NM_004235

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance