KLF4 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724292

This Recombinant KLF4 protein is expressed in HEK-293 Cells and has been mentioned in 23+ publications.

Quick Overview for KLF4 Protein (Myc-DYKDDDDK Tag) (ABIN2724292)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This KLF4 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human KLF4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Soufi, Garcia, Jaroszewicz, Osman, Pellegrini, Zaret: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." in: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).

Verrier, Kochanek, Jackson: "Schwann Cells Metabolize Extracellular 2',3'-cAMP to 2'-AMP." in: The Journal of pharmacology and experimental therapeutics, Vol. 354, Issue 2, pp. 175-83, (2015) (PubMed).

Miyaji, Shahrizaila, Umapathi, Chan, Hirata, Yuki: "Are ERM (ezrin/radixin/moesin) proteins targets for autoantibodies in demyelinating neuropathies?" in: Human immunology, Vol. 75, Issue 11, pp. 1089-91, (2015) (PubMed).

Venkatesh, Johung, Caretti, Noll, Tang, Nagaraja, Gibson, Mount, Polepalli, Mitra, Woo, Malenka, Vogel, Bredel, Mallick, Monje: "Neuronal Activity Promotes Glioma Growth through Neuroligin-3 Secretion." in: Cell, Vol. 161, Issue 4, pp. 803-16, (2015) (PubMed).

He, Schepmoes, Shi, Wu, Fillmore, Gao, Smith, Qian, Rodland, Liu, Camp, Rastogi, Tan, Yan, Mohamed, Huang, Banerjee, Kagan, Srivastava, McLeod, Srivastava, Petrovics, Dobi, Srinivasan: "Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods." in: Journal of translational medicine, Vol. 13, pp. 54, (2015) (PubMed).

Ascione, Rowlinson, Avolio, Katare, Meloni, Spencer, Mangialardi, Norris, Kränkel, Spinetti, Emanueli, Madeddu: "Migration towards SDF-1 selects angiogenin-expressing bone marrow monocytes endowed with cardiac reparative activity in patients with previous myocardial infarction." in: Stem cell research & therapy, Vol. 6, pp. 53, (2015) (PubMed).

Lin, Sivakumaran, Jones, Li, Harper, Wei, Jin, Rustanti, Meunier, Spann, Harrich: "A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1." in: Retrovirology, Vol. 11, pp. 121, (2015) (PubMed).

Shi, Chiang, Labhart, Zhao, Yang, Mistretta, Henning, Maity, Mori-Akiyama: "Context-specific role of SOX9 in NF-Y mediated gene regulation in colorectal cancer cells." in: Nucleic acids research, Vol. 43, Issue 13, pp. 6257-69, (2015) (PubMed).

Kato, Bhattaram, Penzo-Méndez, Gadi, Lefebvre: "SOXC Transcription Factors Induce Cartilage Growth Plate Formation in Mouse Embryos by Promoting Noncanonical WNT Signaling." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 30, Issue 9, pp. 1560-71, (2015) (PubMed).

Planque, Nishiyama, Sonoda, Lin, Taguchi, Hara, Kolodziej, Mitsuda, Gonzalez, Sait, Fukuchi, Massey, Friedland, ONuallain, Sigurdsson, Paul: "Specific amyloid β clearance by a catalytic antibody construct." in: The Journal of biological chemistry, Vol. 290, Issue 16, pp. 10229-41, (2015) (PubMed).

Školáková, Foldynová-Trantírková, Bednářová, Fiala, Vorlíčková, Trantírek: "Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA." in: Nucleic acids research, Vol. 43, Issue 9, pp. 4733-45, (2015) (PubMed).

Park, Kang, Shin, Seo, Kim, Suh, Bae, Shin: "Identification of novel phosphatidic acid-binding proteins in the rat brain." in: Neuroscience letters, Vol. 595, pp. 108-13, (2015) (PubMed).

Boxer, Barajas, Tao, Zhang, Khavari: "ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes." in: Genes & development, Vol. 28, Issue 18, pp. 2013-26, (2014) (PubMed).

von Roemeling, Radisky, Marlow, Cooper, Grebe, Anastasiadis, Tun, Copland: "Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4." in: Cancer research, Vol. 74, Issue 17, pp. 4796-810, (2014) (PubMed).

Adini, Adini, Bazinet, Watnick, Bielenberg, DAmato: "Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, (2014) (PubMed).

Signorile, Petraglia, Baldi: "Anti-mullerian hormone is expressed by endometriosis tissues and induces cell cycle arrest and apoptosis in endometriosis cells." in: Journal of experimental & clinical cancer research : CR, Vol. 33, pp. 46, (2014) (PubMed).

Walpole, Farrell, McGrane, Stewart: "Expression and localization of a UT-B urea transporter in the human bladder." in: American journal of physiology. Renal physiology, Vol. 307, Issue 9, pp. F1088-94, (2014) (PubMed).

Koo, Chae, Kim, Kang, Kim, Kwak, Jung, Cho, Choi, Park, Shin, Jang, Shin, Hwang, Yi, Park: "Identification of novel autoantibodies in type 1 diabetic patients using a high-density protein microarray." in: Diabetes, Vol. 63, Issue 9, pp. 3022-32, (2014) (PubMed).

Lawrence, Conderino, Rieder: "Redistribution of demethylated RNA helicase A during foot-and-mouth disease virus infection: role of Jumonji C-domain containing protein 6 in RHA demethylation." in: Virology, Vol. 452-453, pp. 1-11, (2014) (PubMed).

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Target Details

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: Klf4

Background: This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 49.9 kDa

NCBI Accession: NP_004226

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance