APEX1 Protein (AA 1-318) (GST tag)
(1 reference)-
- Target See all APEX1 Proteins
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-318
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This APEX1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- APEX1 (Human) Recombinant Protein (P01)
- Sequence
- MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD QKTSPSGKPA TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS ENKLPAELQE LPGLSHQYWS APSDKEGYSG VGLLSRQCPL KVSYGIGEEE HDQEGRVIVA EFDSFVLVTA YVPNAGRGLV RLEYRQRWDE AFRKFLKGLA SRKPLVLCGD LNVAHEEIDL RNPKGNKKNA GFTPQERQGF GELLQAVPLA DSFRHLYPNT PYAYTFWTYM MNARSKNVGW RLDYFLLSHS LLPALCDSKI RSKALGSDHC PITLYLAL
- Characteristics
- Human APEX1 full-length ORF ( AAH02338, 1 a.a. - 318 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 97-195) protein (His-IF2DI Tag)
APEX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) protein (His tag)APEX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) protein (T7 tag)APEX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 32-318) protein (His tag)APEX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (Transcript Variant 1) proteinAPEX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Bcl2 inhibits abasic site repair by down-regulating APE1 endonuclease activity." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 9925-32, (2008) (PubMed).
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: "Bcl2 inhibits abasic site repair by down-regulating APE1 endonuclease activity." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 9925-32, (2008) (PubMed).
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- Target
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- Alternative Name
- APEX1 (APEX1 Products)
- Background
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Full Gene Name: APEX nuclease (multifunctional DNA repair enzyme) 1
Synonyms: APE,APE-1,APE1,APEN,APEX,APX,HAP1,REF-1,REF1 - Gene ID
- 328
- Pathways
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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