CDC25A Protein (AA 1-525) (His tag)

Catalog No. ABIN1474609

Product Details

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Protein Type: Recombinant

Protein Characteristics: AA 1-525

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This CDC25A protein is labelled with His tag.

Application: ELISA

Sequence: MELGPEPPHR RRLLFTCSPT PAPQPTGKVQ FGASRAGGLS PVTNLTVTMD QLEGLGSDYE KPMDVRNSSS LQRMGSSEST DSGFCLDSPG PLDSKENLEI SLRRINCLPQ KLLGCSPALK RSHSDSLDHD IFQLIDQDEN KENEAFEFKK PIRPASRGCL NAHVHEESKD PFTHRQNSAP ARMLSSNESD ISESGNFSPL FTPQSPVKAS LSDEDDGFID LLDGENLKND EETPSCMSSL WTAPLVMRRP TNLADRCGLF DSPSPCSSTS SCSTRAVKRA DRSHEESPRG TKRRKSSEAS PVKADVPEPT QLPHQSLSLT SFPKGTIENI FHSDPRDLIG DFSKGYLFHT VSGKHQDLKY ISPEIMASVL NGKFANLIKE FVIIDCRYPY EYEGGHIKGA VNLHMEEEVE EFLLKKPIVP ADGKRVIVVF HCEFSSERGP RMCRYVRERD RLGNEYPKLH YPELYVLKGG YKEFFLKCQS HCEPPSYRPM HHEDFKEDLK KFRTKSRTWA GEKSKREMYS RLKKL

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: M-phase inducer phosphatase 1 (Cdc25a) (CDC25A Products)

Background: Recommended name: M-phase inducer phosphatase 1.
EC= 3.1.3.48.
Alternative name(s): Dual specificity phosphatase Cdc25A

UniProt: P48965

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase