DROSHA Protein (AA 28-160) (His tag)
Quick Overview for DROSHA Protein (AA 28-160) (His tag) (ABIN1691358)
Target
See all DROSHA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 28-160
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Purification tag / Conjugate
- This DROSHA protein is labelled with His tag.
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Purpose
- Recombinant Human Ribonuclease 3/RNASE3 (C-6His)
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Sequence
- RPPQFTRAQW FAIQHISLNP PRCTIAMRAI NNYRWRCKNQ NTFLRTTFAN VVNVCGNQSI RCPHNRTLNN CHRSRFRVPL LHCDLINPGA QNISNCRYAD RPGRRFYVVA CDNRDPRDSP RYPVVPVHLD TTIVDHHHHH H
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Characteristics
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Recombinant Human Ribonuclease 3/RNASE3 (C-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Drosha, Ribonuclease Type III (DROSHA) (AA 1-1374) protein (His tag)
custom-made DROSHA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Drosha, Ribonuclease Type III (DROSHA) (AA 1-1373) protein (His tag)custom-made DROSHA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Drosha, Ribonuclease Type III (DROSHA) (AA 1099-1196) protein (GST tag)DROSHA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mMDTT,10 % Glycerol, pH 7.5.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- DROSHA (Drosha, Ribonuclease Type III (DROSHA))
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Alternative Name
- Ribonuclease 3
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Background
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Recombinant Human Ribonuclease 3/RNASE3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg28-Ile160) of Human RNASE3 fused with a 6His tag at the C-terminus.
Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. -
Molecular Weight
- 16.6 kDa
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UniProt
- P12724
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Pathways
- Regulatory RNA Pathways
Target
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