DROSHA Protein (AA 28-160) (His tag)
DROSHA
Origin: Human
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target See all DROSHA Proteins
- DROSHA (Drosha, Ribonuclease Type III (DROSHA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-160
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This DROSHA protein is labelled with His tag.
- Purpose
- Recombinant Human Ribonuclease 3/RNASE3 (C-6His)
- Sequence
- RPPQFTRAQW FAIQHISLNP PRCTIAMRAI NNYRWRCKNQ NTFLRTTFAN VVNVCGNQSI RCPHNRTLNN CHRSRFRVPL LHCDLINPGA QNISNCRYAD RPGRRFYVVA CDNRDPRDSP RYPVVPVHLD TTIVDHHHHH H
- Characteristics
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Recombinant Human Ribonuclease 3/RNASE3 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product DROSHA Protein
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Drosha, Ribonuclease Type III (DROSHA) (AA 1099-1196) protein (GST tag)
DROSHA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mMDTT,10 % Glycerol, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- DROSHA (Drosha, Ribonuclease Type III (DROSHA))
- Alternative Name
- Ribonuclease 3 (DROSHA Products)
- Synonyms
- im:7150667 Protein, zgc:158612 Protein, ETOHI2 Protein, HSA242976 Protein, RANSE3L Protein, RN3 Protein, RNASE3L Protein, RNASEN Protein, 1110013A17Rik Protein, AI874853 Protein, Etohi2 Protein, Rn3 Protein, Rnasen Protein, RGD1307626 Protein, ribonuclease type III, nuclear Protein, Ribonuclease 3 Protein, ribonuclease 3 Protein, drosha ribonuclease III Protein, drosha, ribonuclease type III Protein, rnasen Protein, Thicy_0885 Protein, Sinme_0866 Protein, Isova_2087 Protein, Sphch_0770 Protein, DROSHA Protein, Drosha Protein
- Background
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Recombinant Human Ribonuclease 3/RNASE3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg28-Ile160) of Human RNASE3 fused with a 6His tag at the C-terminus.
Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. - Molecular Weight
- 16.6 kDa
- UniProt
- P12724
- Pathways
- Regulatory RNA Pathways
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