Recombinant Human Galectin-Related Protein/LGALSL is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Ser172) of Human LGALSL fused with a 6His tag at the C-terminus.
Purity
> 95 % as determined by reducing SDS-PAGE.
Sterility
0.2 μm filtered
Endotoxin Level
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
GRP
Reactivity: Human
Source: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Crys, ELISA, SDS, WB
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM Tris, 100 mM NaCl, pH 8.0.
Handling Advice
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Storage
-80 °C
Storage Comment
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Galectin-Related Protein (LGALSL) is a 172 amino acid protein that contains one Galectin domain. LGALSL does not appear to bind carbohydrates or lactose as the critical residues required for binding are not conserved. LGALSL may play a significant role in stimulating smooth muscle growth in developing alveolar wall vessels and the development of pulmonary capillaries. Alternative Names: Galectin-Related Protein, Lectin Galactoside-Binding-Like Protein, LGALSL, GRP, HSPC159