GOT1 Protein (AA 2-413) (His tag)
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- Target See all GOT1 Proteins
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-413
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Origin
- Human
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Source
- Yeast (Pichia pastoris)
- Purification tag / Conjugate
- This GOT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Sequence
- YVEFHHHHHH -APPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
- Characteristics
- Location of tag(s): N-terminal
- Purity
- > 95 %
- Top Product
- Discover our top product GOT1 Protein
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Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) protein (GST tag)
GOT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)GOT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Comment
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Subcellular Location: Cytoplasm
Predicted Isoelectric Point: 6.6
The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 °C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile PBS, pH 7.2 - pH 7.4.
- Buffer
- Supplied as lyophilized form in PBS, pH 7.4, containing 5 % trehalose, 0.05 % sarcosyl.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Alternative Name
- Aspartate Aminotransferase (AST) (GOT1 Products)
- Molecular Weight
- 47.5 kDa
- UniProt
- P17174
- Pathways
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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